Part:BBa_J37016:Design

Predator Molecule Generator Sensing Test (GFP output)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1532
  

Design Notes

• The part is designed to produce both LuxR and GFP in response to different AHL concentrations.

with LuxI under the control of Lux pR *LuxR and GFP are under the control of the promoter luxpR, which is activated by the heterodimer AHL/LuxR

• The part has been ligated from the following previous parts: BBa_J37019,

J37025*Expression of GFP downstream of the promoter serves as an indicator of the production of LuxR.

• More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct/Design here].

Source

na